Produktprisbilaga. Karolinska Institutet Upphandling 8050/UF Molekylärbiologi SIGMA-ALDRICH Rev - PDF

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1 Restriktions endonukleaser: 1.1 Aat II 1.2 Acc I 100UN 5,000 units/ml R UN Sigma-Aldrich 744 7, Afl III 1.4 Age I 1.5 Alw I 1.6 AlwN I 1.7 Apa I 2000UN 10,000 units/ml R4258-2KU Sigma-Aldrich

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1 Restriktions endonukleaser: 1.1 Aat II 1.2 Acc I 100UN 5,000 units/ml R UN Sigma-Aldrich 744 7, Afl III 1.4 Age I 1.5 Alw I 1.6 AlwN I 1.7 Apa I 2000UN 10,000 units/ml R4258-2KU Sigma-Aldrich 293 0, ApaL I 1.9 Asc I 1.10 Ase I 1.11 Asp Ava I 250UN 5,000 units/ml R UN Sigma-Aldrich 480 1, Ava II 100UN 5,000 units/ml R UN Sigma-Aldrich 193 1, BamH I 1000UN 10,000 units/ml R0260-1KU Sigma-Aldrich 146 0, BamH I 5000UN 10,000 units/ml R0260-5KU Sigma-Aldrich 285 0, Ban II 1.16 BcLO 1.17 Bgl I 250UN 10,000 units/ml R UN Sigma-Aldrich 158 0, Bgl II 100UN 10,000 units/ml R UN Sigma-Aldrich 96 0, Bgl II 500UN 10,000 units/ml R UN Sigma-Aldrich 241 0, BSMB I 1.20 Bst XI 1.21 Cla I 500UN 10,000 units/ml R UN Sigma-Aldrich 439 0, Cvi AII 1.23 Dde I 200UN 10,000 units/ml R UN Sigma-Aldrich 235 1, Dpn I 200UN 10,000 units/ml R UN Sigma-Aldrich 453 2, Dra I 2000UN 10,000 units/ml R4381-2KU Sigma-Aldrich 473 0, Dra III 1.27 Eae I 1.28 Eag I 1.29 EcoN I 1.30 EcoR V 1000UN 10,000 units/ml R2756-1KU Sigma-Aldrich 166 0, EcoRI 1000UN 10,000 units/ml R6265-1KU Sigma-Aldrich 99 0, EcoRI 5000UN 10,000 units/ml R6265-5KU Sigma-Aldrich 207 0, EcoRI high conc. 1000UN 40,000 units/ml R4640-1KU Sigma-Aldrich 113 0, EcoRI high conc. 5000UN 40,000 units/ml R4640-5KU Sigma-Aldrich 219 0, Fse I 1.34 Fsp I 1.35 Hae II 1.36 Hae III 1000UN 10,000 units/ml R5628-1KU Sigma-Aldrich 165 0, Hae III 2500UN 10,000 units/ml R KU Sigma-Aldrich 411 0, Hinc II 1.38 Hind III 1000UN 10,000 units/ml R1137-1KU Sigma-Aldrich 63 0, Hind III 5000UN 10,000 units/ml R1137-5KU Sigma-Aldrich 179 0, Hpa I 500UN 3,000-10,000 units/ml R UN Sigma-Aldrich , Hpa II 500UN 10,000 units/ml R UN Sigma-Aldrich 304 0, Kas I 1.42 Kpn I 1000UN 10,000 units/ml R1258-1KU Sigma-Aldrich 214 0, Mfe I 1.44 Mlu I 500UN 10,000 units/ml R UN Sigma-Aldrich 315 0, MscI 1.46 Msp I 2000UN 10,000 units/ml R4506-2KU Sigma-Aldrich 608 0, Nae I 1.48 Nar I 1.49 Nci I 1.50 Nco I 100UN 10,000 units/ml R UN Sigma-Aldrich 320 3, Nde I 200UN 10,000 units/ml R UN Sigma-Aldrich 428 2, Nhe I 500UN 10,000 units/ml R UN Sigma-Aldrich 946 1, Not I 1.54 Nsi I 1000UN 10,000 units/ml R5884-1KU Sigma-Aldrich , Pac I 1.56 Pin A I 1.57 Pme I 1.58 Pst I 100UN 10,000 units/ml R UN Sigma-Aldrich 101 1, 1.59 Pst I 500UN 10,000 units/ml R UN Sigma-Aldrich 189 0, Pst I high conc Pvu I 50UN 5,000 units/ml R UN Sigma-Aldrich 270 5, Pvu II 1000UN 10,000 units/ml R2631-1KU Sigma-Aldrich 231 0, Rsa I 1000UN 10,000 units/ml R4756-1KU Sigma-Aldrich 383 0, Sac I 1000UN 10,000 units/ml R5268-1KU Sigma-Aldrich 394 0, Sac II 1.66 Sal I 1000UN 10,000 units/ml R0754-1KU Sigma-Aldrich 266 0, Sca I 1000UN 10,000 units/ml R5007-1KU Sigma-Aldrich 529 0, Sfi I 500UN 10,000 units/ml R UN Sigma-Aldrich 405 0, Sma I 2500UN 10,000 units/ml R KU Sigma-Aldrich 921 0, Snab I 1.71 Spe I 150UN 10,000 units/ml R UN Sigma-Aldrich 284 1, Sph I 100UN 10,000 units/ml R UN Sigma-Aldrich 494 4, Ssp I 200UN 10,000 units/ml R UN Sigma-Aldrich 394 1, Stu I 1.75 Tth111 I 1.76 Xba I 2000UN 10,000 units/ml R7260-2KU Sigma-Aldrich 275 0, Xcm I 1.78 Xho I 2500UN 10,000 units/ml R KU Sigma-Aldrich 286 0, Xma I 1.80 Xmn I Rabattsats för andra restriktionsenzymer:0% 2 Polymeraser, transferaser, transkriptaser, kinaser 2.1 Heatstable proof reading polymerase 500UN 5 units/μl D UN Sigma-Aldrich , Heatstable DNA polymeras 250UN 2.5 units/μl D UN Sigma-Aldrich 940 3, DNA polymerase I (E.Coli) 2000UN 5,000-15,000 units/ml D9380-2KU Sigma-Aldrich , DNA polymerase I, (Klenow fragment) 100UN ~3,000 units/ml D UN Sigma-Aldrich 460 4, T4 DNA polymerase 50UN 5,000-20,000 units/ml D UN Sigma-Aldrich , T7 DNA polymerase 2.7 Klenow fragment 100UN ~3,000 units/ml D UN Sigma-Aldrich 460 4, Klenow fragment 3-5 exo 100UN ~3,000 units/ml D UN Sigma-Aldrich 460 4, SP6 RNA polymerase 1000UN 10,000-50,000 units/ml R0759-1KU Sigma-Aldrich 550 0, T7 RNA polymerase 1000UN 10,000-50,000 units/ml R0884-1KU Sigma-Aldrich 374 0, T3 RNA polymerase 2.12 PolyA polymerase ingen info STR1-1KT Sigma-Aldrich 2122 n/a Phusion high fidality DNA polymerase 125UN 5 units/μl D UN Sigma-Aldrich 438 3, phi29 DNA polymerase 2.15 Terminal transferase 300UN 5,000 units/ml T UN Sigma-Aldrich 661 2, Methyltransferase 2.17 Topoisomerase 1 / Cre recombinase 1000UN ingen info T UN Sigma-Aldrich , Reversed transcriptase M-MuLV 40000UN 200 units/μl M KU Sigma-Aldrich , T4 Polynucleotide kinase 500UN ~10 units/μl P UN Sigma-Aldrich , Uracil-DNA glycosylase 2.21 Alkaline phosphatase (Calf/shrimp/arctic) 12500UN ~2500 units/mg MG Sigma-Aldrich , A/G-specific adenine glycosylase MutY 3 Ligaser 3.1 E.Coli DNA ligase 3.2 T4 DNA ligase 500UN 500-6,000 units/ml D UN Sigma-Aldrich , T4 RNA ligase 3.4 Taq DNA ligase 3.5 Thermostable DNA ligase 4 DNA Nukleaser 4.1 Exonuclease I (E.Coli) 4.2 Exonuclease III (E.Coli) 5000UN U/μL E1131-5KU Sigma-Aldrich 335 0, Exonuclease T 4.4 Lambda Exonuclease 4.5 T7 Endonuclease I 4.6 Endonuclease III 100UN =10,000 units/mg E UG Sigma-Aldrich , Endonuclease IV 4.8 Mung Bean Nuclease 4.9 T7 Exonuclease 4.10 T7 Endonuclease 4.11 RNase free DNase UN 5,000 units/mg D5319-2MG Sigma-Aldrich , S1 Nuclease 5 Rnaser 5.1 RNase H 5.2 RNase A 3500UN 70 Kunitz units/mg R MG Sigma-Aldrich 682 0, RNase I 5.4 RNase III 5.5 RNase Inhibitor 10000UN 30,000-50,000 units/ml R KU Sigma-Aldrich , Proteaser 6.1 Proteinase K 100mg 100mg P MG Sigma-Aldrich , Proteinase K 1g 1g P2308-1G Sigma-Aldrich , Proteinase K 20mg/ml 5ml 1ml 500 units/ml P5568-1ML Sigma-Aldrich ,40 0, DNA-RNA & Protein storleksmarkörer bp DNA Step Ladder bp DNA Step Ladder 40UG 80 uses P UG Sigma-Aldrich , bp DNA Step Ladder 50UG S UG Sigma-Aldrich 305 6, bp DNA Step Ladder 1VL P1473-1VL Sigma-Aldrich kb DNA Step Ladder 1VL D0428-1VL Sigma-Aldrich bp DNA Ladder 1VL P1473-1VL Sigma-Aldrich bp DNA ladder 250UG D UG Sigma-Aldrich , kb DNA Ladder 1VL 100 uses D0428-1VL Sigma-Aldrich MicroRNA Ladder 7.10 RNA Ladder Single stranded baser 50UG 25 uses R UG Sigma-Aldrich , RNA Ladder Single stranded baser 1VL 25 uses R7644-1VL Sigma-Aldrich RNA Ladder Single stranded baser 50UG 25 uses R UG Sigma-Aldrich , RNA Ladder Double stranded 7.14 RNA Molecular Weight Marker 5UG R4142-5UG Sigma-Aldrich , Lambda DNA markers 0,5MG D MG Sigma-Aldrich , Unstained protein markers kDa 10VL S VL Sigma-Aldrich Prestained protein markers kda 1VL C1992-1VL Sigma-Aldrich PCR molecular markers 1VL 7.19 pbr322 DNA -BstN I digest marker 7.20 pbr322 DNA -Msp I digest marker 7.21 TAMRA size standard TAMRA size standard LIZ size standard Lambda DNA 0,1MG D MG Sigma-Aldrich , Lambda DNA (N6-methyladenine-free) Andra rekommenderade storleksmarkörer Rabattsats för andra storleksmarkörer:0 % 8 Nukleotider, primrar, mastermix 8.1 (dntp)set 10mM 1KIT DNTP10-1KT Sigma-Aldrich (dntp)set 100mM 1KIT DNTP100-1KT Sigma-Aldrich NTP mix 10mM ~0.5 mg/ml 100 uses D ML Sigma-Aldrich , Syber green master mix 1.25 units/reactio S RXN Sigma-Aldrich Universal PCR master mix 1.5 units/reaction P RXN Sigma-Aldrich Universal PCR master mix without UNG 8.7 Sequencing primers T7, T3, M13 and others T7 primer set P7348-1SET Sigma-Aldrich M13 Forward primp2973-1set Sigma-Aldrich M13 Reverse primp3098-1set Sigma-Aldrich Oligo(dT) primers 8.9 Random primers H0268-1UN Sigma-Aldrich Standard linkers for various restriction enzyme 8.10 sites w or w/o phosphorylation 50 rxn LIG2-1KT Sigma-Aldrich Ribosomal RNA control reagent DNA solutions for hyridization (herring sperm, 8.12 salmon sperm, calf thymus) Herring sperm D7290-5X1ML Sigma-Aldrich , Salmon sperm G-F Sigma-Aldrich 439 8, Calf thymus D4764-5UN Sigma-Aldrich , Kloner 9.1 Full length mouse cdna (verified) 9.2 Full length human cdna (verified) 9.3 Full length chicken cdna (verified) 9.4 Mouse EST clones 9.5 Human EST clones 9.6 Chicken EST clones 9.7 Mouse PAC clones 9.8 Human PAC clones 9.9 Mouse BAC clones 9.10 Human BAC clones Andra rekommenderade kloner Rabattsats för andra kloner:0% 10 Genbibliotek 10.1 Mouse cdna library 10.2 Human cdna library 10.3 Chicken cdna library 10.4 PAC libraries 10.5 BAC libraries Andra rekommenderade genbibliotek Rabattsats för andra genbibliotek:0% 11 Kompetenta celler 11.1 Bakteria, various strains including dam/dcm-bugs 11.2 Chemically competent E.coli J X50UL Sigma-Aldrich , Andra rekommenderade kompetenta celler Rabattsats för andra kompetenta celler:0% 12 Molecular grade agaros 15 Polyacrylamid gel electrophores (PAGE) reagenser och färdiga geler 15.1 acrylamide 500G A G Sigma-Aldrich 265 0, arylamide färdig lösning 100ML A ML Sigma-Aldrich 79 0, bis 100G M G Sigma-Aldrich 199 1, TEMED 100ML T ML Sigma-Aldrich 390 3, ammonium persulfate 100G A G Sigma-Aldrich 58 0, Bis-Tris 10%, 1,0 mm, färdig gel 15.3 Bis-Tris 10%, 1,5 mm, färdig gel 15.4 Bis-Tris 12%, 1,0 mm, färdig gel 15.5 Bis-Tris 4-12%, 1,0 mm, färdig gel 15.6 Bis-Tris 4-12%, 1,5 mm, färdig gel 15.7 SDS Running Buffer for Bis-Tris gels 1L 10 concentrate 10x1L L Sigma-Aldrich 675 0, Tris-Glycine 4-20%, 1,5 mm, färdig gel Andra rekommenderade färdiga geler Rabattsats för andra färdiga geler:0% 16 Blotting membran, film 16.1 PVDF, 02 um 10EA 10 Z EA Sigma-Aldrich Nitrocellulosa p y p g 5EA 5 N7892-5EA Sigma-Aldrich (NC 0.2 um, PVDF 0.2 um) 100EA 100 P EA Sigma-Aldrich detection, 18x24 cm 17 Kolonner 17.1 Size exclusion spin columns for nucleic acids (salt removal) 20EA 20 S EA Sigma-Aldrich Size exclusion spin columns for proteins 1KT SC1000-1KT Sigma-Aldrich IMAC columns 25EA 25 H EA Sigma-Aldrich Spin columns for cell-lysate homogenization 1KT G1N70-1KT Sigma-Aldrich Reversed phase columns for concentration of peptide solutions 18 Dialysslangar och kassetter 18.1 Dialysslang 6-8 kd 18.2 Dialysslang kd 18.3 Kassett 2-3 kd 18.4 Kassett kd Rabattsats för andra dialysslangar och kassetter:0% 19 Optiska plattor 19.1 Optical 96-well plates, covers/caps 100EA 100 CLS EA Sigma-Aldrich Adhesive plate sealers 100EA 100 CLS EA Sigma-Aldrich Övrigt 20.1 Dextran sulphate sodium 50G D G Sigma-Aldrich 435 8, Ethidium bromide 5G E7637-5G Sigma-Aldrich , Isopropyl β-d-1-thiogalactopyranoside (IPTG) 5G I6758-5G Sigma-Aldrich , MG-132 Proteasome inhibitor 20.5 Luria Broth Base 1KG L1900-1KG Sigma-Aldrich 894 0, Nuclease free water 1L W4502-1L Sigma-Aldrich 120 0, DEPC treated water 1L L Sigma-Aldrich 260 0, Highly deionized formamide 100ML F ML Sigma-Aldrich 152 1, HRP-labeled y p protein g A ( µ ) controlled amount of carboxylic acid groups on kan ej erbjuda ProteinG Magnetic Beads for immunoprecipitation kan ej erbjuda Surface decontaminants (nuclease and DNA) 250ML L ML Sigma-Aldrich 123 0, Rabattsats för andra molekylärbiologiska produkter, utöver de redan rabatterade produktgrupperna:0% Snabbleverans i SEK eller : se bilaga PS-ODR-ALL-0005 Fraktkostnader.pdf Specialleverans i SEK eller : se bilaga PS-ODR-ALL-0005 Fraktkostnader.pdf OBS!!! ska kit, se blad 2 nedan. MOLEKYLÄRBIOLOGISKA KIT OCH KIT FÖR RENING AV NUKLEINSYROR Bilaga Antal reaktioner, Förpackningsstorlek Koncentration Pris/isolering,analys, Pris/ Pos Produkt analyser,isoleringar/kit (µg/mg/g/µl,ml,l) (enhet) Katalognr Tillverkare Pris/fp,kit reaktion µg/mg/g/µl,ml,l 21 ska kit 21.1 Cloning kit for Expression 25 reaktioner RDC1-1KT Sigma-Aldrich Cloning kit for Seqencing 25 reaktioner RDC1-1KT Sigma-Aldrich Cloning kit for blunt end PCR products 25 reaktioner RDC1-1KT Sigma-Aldrich Cloning kit for A/T overhang PCR 25 reaktioner RDC1-1KT Sigma-Aldrich First strand cdna Synthesis kit 50 reaktioner STR1-1KT Sigma-Aldrich Double strand cdna Synthesis kit 21.7 Real Time PCR kit 400 reaktioner D RXN Sigma-Aldrich , qpcr kit (SYBR and probe detection) 480 detektions reaktioner PPD1-1KT Sigma-Aldrich , Quick mini-scale DNA amplification kit 4-7 µg 100 amplifikationer, 100 extraktioner XNAT2-1KT Sigma-Aldrich Gene expression kit 9 komponenter FLMC-1KT Sigma-Aldrich Extended-PCR kit Reverse Transcriptase PCR kit 50 reaktioner STR1-1KT Sigma-Aldrich MicroRNA Reverse Transcription kit DNA Ligation kit 150 ligerings reaktioner LIG1-1KT Sigma-Aldrich , In vitro Mutagenes kit (site directed mutagenes) Single Nucleotide Polymorphism (SNP) Kits Genotyping assays PolyA spin mrna isolation kit 70 purifcations MRN70-1KT Sigma-Aldrich In vitro Transcription kit sp units 10,000-50,000 units/ml R0759-1KU Sigma-Aldrich 550 0,55 /unit In vitro Transcription kit T In vitro Transcription kit T units 10,000-50,000 units/ml R0884-1KU Sigma-Aldrich 374 0,37 /unit In vitro Translation kit 5 komponenter TN0100-1KT Sigma-Aldrich Transfection kit / reagents/lipofectamine/plus reagent 0,5 ml 2 mg/ml E ML Sigma-Aldrich /ml Nucleofection kit RNAi transfection kit / reagents 400 µl N UL Sigma-Aldrich ,68/ µl RNAi kits Nick Translation kit 2 mg D5319-2MG Sigma-Aldrich /mg Random primer labeling kit 50 st R EA Sigma-Aldrich DIG-labeling kit Multicolor labeling kit Sequencing kit 4 ml S3938-4X1ML Sigma-Aldrich /ml Purification kit for DNA sequencing reactions (96-well plate) 2 st plattor S4309-2EA Sigma-Aldrich 821 4, Reporter gene assay using luciferase 100 assays LUC1-1KT Sigma-Aldrich 972 9, Reporter gene assay using B-galactosidase 65 test GALA-1KT Sigma-Aldrich , ATP substrate kit 3 komponenter FLAA-1KT Sigma-Aldrich Silver staining kit för 25 mini geler PROTSIL1-1KT Sigma-Aldrich , Western Blot Chemiluminescent detection reagent N/A RACE-kit Kit for protein quantification (no BCA protein assay kits) B ML Sigma-Aldrich 370 0,74 /ml Bile acid kit 5 komponenter BA-1KT Sigma-Aldrich 468 Andra rekommenderade mol.biologiska kit: Rabattsats för andra molekylärbiologiska kit:0% 22 DNA reningskit: 22.1 Gel extraction 70 reningar NA1111-1KT Sigma-Aldrich 692 9, Nucleotide removal 70 reningar NA1020-1KT Sigma-Aldrich 592 8, Genomic DNA from blood 70 reningar NA2010-1KT Sigma-Aldrich , Genomic DNA purification reagents for high volyme samples Genomic DNA from blood, up to 100 ug, up to 5 ml blood 70 reningar NA2010-1KT Sigma-Aldrich , Genomic DNA purification reagents for high volyme samples for automated systems (Autopure or similar) Cell lysis solution 250 ml C ML Sigma-Aldrich 793 3,17 /ml RBC lysis solution 100 ml R ML Sigma-Aldrich 132 1,32 /ml Rnase A solution 50 mg R MG Sigma-Aldrich ,6 /mg DNA hydration solution Protein precipitation solution 3 komponenter PROTPR-1KT Sigma-Aldrich DNA isolation from stool 22.6 DNA isolation from various biol. Samples 200 ml T ML Sigma-Aldrich ,59 /ml 22.7 Lambda DNA 22.8 Plasmid kit Mini, ange bindningskapacitet 70 reningar PLN70-1KT Sigma-Aldrich 462 6, Plasmid kit Midi, ange bindningskapacitet 35 reningar PLD35-1KT Sigma-Aldrich Plasmid kit Maxi, ange bindningskapacitet 15 reningar PLX15-1KT Sigma-Aldrich Plasmid kit Giga, ange bindningskapacitet 5 reningar NA0800-1KT Sigma-Aldrich EndoFree Plasmid kit Maxi 10 preparationer NA0400-1KT Sigma-Aldrich Genomic DNA Buffer Kit PCR purification kit. DNA range 100bp-5kb 70 reningar NA1020-1KT Sigma-Aldrich 592 8, PAC/BAC clone isolation kits Magnetic bead isolation kits for cells Andra rekommenderade DNA reningskit: Rabattsats för andra DNA reningskit:0% 23 RNA reningskit: 23.1 mrna from biol.samples 70 reningar MRN70-1KT Sigma-Aldrich mrna from total RNA 70 reningar MRN70-1KT Sigma-Aldrich Total RNA from biol. Samples 70 reningar RTN70-1KT Sigma-Aldrich mrna kits tested for ChIP/ChIP-onChIP/arrays/deep sequencing assays MOLEKYLÄRBIOLOGISKA KIT OCH KIT FÖR RENING AV NUKLEINSYROR 23.5 total RNA kits tested for ChIP/ChIP-onChIP/arrays/deep sequencing assays 23.6 Magnetic bead RNA isolation kit 23.7 RNA stabilization reagent Andra rekommenderade RNA reningskit: Rabattsats för andra RNA reningskit:0% Bilaga Vektorer: 24.1 Vectors for Cloning 24.2 Vectors for sirna cloning 24.3 Vectors for Sequencing 24.4 Vectors for Eucaryotic expression 24.5 Vectors for Procaryotic expression 24.6 Lentiviral vector system N/A 24.7 Luciferase detection vector SHC007 Sigma-Aldrich B-galactosidase detection vector 24.9 Fluorescent protein vector Andra rekommenderade vektorer: Rabattsats för andra vektorer:0% Rabattsats för andra molekylärbiologiska produkter, utöver de redan rabatterade produktgrupperna:0% Snabbleverans i SEK eller : se bilaga PS-ODR-ALL-0005 Fraktkostnader.pdf Specialleverans i SEK eller : se bilaga PS-ODR-ALL-0005 Fraktkostnader.pdf
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