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ELENI MYLONA CURRICULUM VITÆ Mailing Address: Foundation for Research and Technology HELLAS (FORTH) Institute of Applied and Computational Mathematics (IACM) Vassilika Vouton Heraklion, Crete HELLAS

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ELENI MYLONA CURRICULUM VITÆ Mailing Address: Foundation for Research and Technology HELLAS (FORTH) Institute of Applied and Computational Mathematics (IACM) Vassilika Vouton Heraklion, Crete HELLAS (Greece) Telephone Number: , Fax Number: Address: Website: EDUCATION Ph.D. (2003) M.S. (1998) B.S. (1996) Diploma (1991) Applied Physiology (major), Department of Kinesiology, The University of Toledo, Toledo, OH, USA Advisor: Dr. Francis X, Pizza Dissertation title: Myotube-derived neutrophil chemoattractants: Effects of mechanical strain and injury. Molecular/Cell Biology (minor), Department of Pathology, Health Science Campus, The University of Toledo, Toledo, OH, USA Advisor: Dr. William T. Gunning, III Exercise Science, Department of Health Promotion and Human Performance, The University of Toledo, Toledo, OH, USA Advisor: Dr. Fredrick F. Andres Thesis title: Effects of a single bout of moderate swimming and running on arterial blood pressure during exercise and recovery . Exercise Science and Physical Education, Department of Physical Education and Sports, Democritus University of Thrace, Komotini, HELLAS Advisor: Dr. Panagiotis Antoniou Thesis title (with honors): Effects of heredity on hemodynamics of pre-adolescent swimmers during maximal exercise and recovery . Physical Education (exchange student), Department of Physical Education, College of St. Mark & St. John, Plymouth, UK, 1995 Advisor: Dr. Harry Richardson Palamaiki Scholi - 1st Geniko Lykeio (High School), Major: Ancient Hellenic Literature, Latin, and History, Messolonghi, HELLAS, 1991 PROFESSIONAL EXPERIENCE present Adjunct t Assistant Professor, University of Maryland University College-Europe, European Headquarters: Heidelberg, GERMANY present Adjunct Research Scientist, Institute of Applied and Computational Mathematics, Foundation for Research and Technology-HELLAS, Heraklion, HELLAS Post-doctoral Fellow, Institute of Applied and Computational Mathematics, Foundation for Research and Technology-HELLAS, Heraklion, HELLAS Post-doctoral Fellow, Department of Pharmacology, Emory University School of Medicine, Atlanta, GA, USA Graduate Teaching/Research Assistant, Department of Kinesiology, The University of Toledo, Toledo, OH, USA Research Assistant (volunteer), Department of Physiology and Pharmacology, Health Science Campus, The University of Toledo, Toledo, OH, USA Instructor, Division of Continuing Education, The University of Toledo, Toledo, Ohio, USA Undergraduate Research Assistant, Applied Physiology Laboratory, Department of Physical Education and Sports, Democritus University of Thrace, Komotini, HELLAS RESEARCH PROJECTS present Mechanotaxis in the guidance of cancer metastasis: Interaction between cancer cells and the mechanical properties of their microenvironment. IACM, FORTH, Heraklion, HELLAS Modeling tumor taxis: migration of breast cancer cells in response to mechanical and chemical stimuli. IACM, FORTH, Heraklion, HELLAS Supervisor: Dr. Daphne Manoussaki Molecular pathways of skeletal muscle cell migration and fusion. Dept. of Pharmacology, Emory University School of Medicine, Atlanta, GA, USA Supervisor: Dr. Grace K. Pavlath Skeletal Muscle Immunobiology During Inflammation. Dept. of Kinesiology, The University of Toledo, Toledo, OH, USA TEACHING EXPERIENCE Graduate Courses MATH131 Biomathematics I Population Biology: Our internal micro-world in kinesis: a special glance on cancer cell metastasis (invited lecture; Technical University of Crete) PHARM/BCDB Supervised doctoral student rotation research (Emory) PHARM 531 KINE 6/8550 Introduction to Pharmacology: Excitation-Contraction Coupling (invited lecture; Emory) Advanced Laboratory Techniques in Exercise Physiology (Laboratory; UT) Page 2 KINE 6/8540 Laboratory Techniques in Exercise Physiology (laboratory; UT) Undergraduate Courses BIOL 103 Introduction to Biology (UMUM-Europe) KINE 3530 KINE 2540 KINE 1080 Other Classes KINE 1/2000 Cont. Education Extracurricular Applied Exercise Physiology Laboratory (UT) Human Physiology Laboratory (UT) Health and Exercise (lecture and laboratory; UT) Sports training/coaching (swimming, water polo, volleyball, tennis; UT) Swimming instructor for infants, toddlers and children (UT) Swimming instructor (Democritus University of Thrace) ACADEMIC HONORS AND AWARDS Outstanding Doctoral Student Award, Department of Kinesiology, The University of Toledo, Toledo, OH, USA Outstanding Graduate Student Award, Department of Health Promotion and Human Performance, The University of Toledo, Toledo, OH, USA Class Valedictorian, Democritus University of Thrace, Komotini, HELLAS Outstanding Student Fellowship, Foundation of State Scholarships, HELLAS Erasmus Exchange Student Scholarship, EUROPEAN UNION (EU) Outstanding Student Fellowship (ranked 1st in class), Foundation of State Scholarships, HELLAS Outstanding Student Fellowship (ranked 1st in class), Foundation of State Scholarships, HELLAS GRANTS Research Principal Investigator, Marie Curie International Reintegration Grant (MCIRG), European Commission FP6, Marie Curie Actions, (80,000 ) Mechanotaxis in the guidance of cancer metastasis Foundation of Research and Technology-HELLAS, Institute of Applied and Computational Mathematics, Heraklion, Crete, HELLAS Post-doctoral Fellow, Muscular Dystrophy Association, Regulation of myoblast migration and fusion by class III semaphorins. Pricipal Investigator: G.K. Pavlath, Emory University School of Medicine, Atlanta, GA, USA Doctoral Student, National Institutes of Health, National Institute of Arthritis and Page 3 Travel Eleni Mylona Curriculum Vitae Musculoskeletal and Skin Diseases (RO3): Injured muscle effect on neutrophils function Principal Investigator: F.X. Pizza, The University of Toledo, Toledo, OH, USA 2006 Young Researchers Travel Award, European Commission FP6 - Marie Curie Actions, Euroconference in Biomathematics, Anogia, Crete, HELLAS Graduate Student Travel Award, The University of Toledo, Toledo, OH, USA 1998 Graduate Student Travel Award, The University of Toledo, Toledo, OH, USA Other Research Grants 2010 Collaborator, Hellenic Ministry of Education & Religions, FP7 Programme Thalis , Nano-engineering in understanding biological functions Status: Pending 2010 Collaborator, Hellenic Ministry of Education & Religions, FP7 Programme Thalis , Biomechanical and systemic analysis of chondron degeneration in arthritis Status: Pending 2009 Principal Investigator, Hellenic Ministry for Development, General Secretariat for Research & Technology, France-Greece Joint Research and Technology Programmes , Analysis of the mechanosensitive adhesion of cancer cells with approaches coupling nanotechnologies and numerical simulation Status: Denied 2008 Principal Investigator, Association for International Cancer Research, Genomic, proteomic, and functional responses of cancer cells to microenvironment mechanics Status: Denied PUBLICATIONS 1. Stéphanou A, E. Mylona, M. Chaplain and P. Tracqui. A computational model of cell migration coupling the growth of focal adhesions with oscillatory cell protrusions. J. Theor. Bio. 253(4): , Mylona, E., K. A. Jones, S.T. Mills, and G.K. Pavlath. CD44 regulates myoblast migration and differentiation J. Cell. Physiol. 209(2): , Tsivitse, S.K.*, E. Mylona*, J.M. Peterson, W.T. Gunning, and F.X. Pizza. Mechanical loading and injury induce human myotubes to release neutrophil chemoattractants. Amer. J. Physiol. Cell Physiol. 288 (3): C721-9, 2005 *equal contribution. 4. McLoughlin, T.J.*, E. Mylona*, T.A. Hornberger, K.A. Esser, F.X. Pizza. Inflammatory cells in rat skeletal muscle are elevated after electrically stimulated contractions. J. Appl. Physiol. 94(3): , 2003 *equal contribution. 5. Peterson, J.M., T.A. Trappe, E. Mylona, F. White, C.P. Lambert, W.J. Evans, F.X. Pizza. Ibuprofen and acetaminophen: effect on muscle inflammation after eccentric exercise. Med. Sci. Sports Exerc. 35(6): 892-6, Page 4 6. Tsivitse, S.K., T.J. McLoughlin, J.M. Peterson, E. Mylona, S.J. McGregor, and F. X. Pizza. Downhill running in rats: influence on neutrophils, macrophages, and MyoD+ cells in skeletal muscle. Eur. J. Appl. Physiol. 90(5-6): 633-8, Mylona, E., M. Fahlman, A.L. Morgan, D.J. Boardley, and S.K. Tsivitse. S-IgA following a single bout of exercise: Effects of fitness level and ambient temperature. Int. J. Sports Med., 23(6): , Frenette, J., M. St-Pierre, C. H. Cote, E. Mylona, and F. X. Pizza. Muscle impairment occurs rapidly and precedes inflammatory cell accumulation after mechanical loading. Amer. J. Physiol., 282: R351- R357, Lambert, C.P., M.G. Flynn, W.A. Braun, and E. Mylona. Influence of acute submaximal exercise on T-lymphocyte suppressor cell function in healthy young men. Eur. J. Appl. Phys., 82 (1/2): , Braun, W.A., M.G. Flynn, D.E. Jacks, T. McLoughlin, J. Sowash, C.P. Lambert, and E. Mylona. Indomethacin does not influence natural cell-mediated cytotoxicity response to endurance exercise. J. Appl. Phys., 87: , PEER EER-REVIEWED EVIEWED ARTICLES IN CONFERENCE PROCEEDINGS 1. Mylona E., Z.H. Dailiana, X. Trepat, and M.G. Lagoudakis. Substrate Rigidity Dictates Phenotype, Survival, and Mechanics of Primary Human Osteosarcoma Cells, 6 th European Symposium on Biomedical Engineering Symposium Proceedings, Jetzek F., E. Mylona, and D. Manoussaki. Prediction of potential locations of focal adhesions on the contour of adherent cells, Fifth IEEE International Symposium on Biomedical Imaging Proceedings, ABSTRACT PUBLICATIONS 1. Mylona E., Alexopoulos L.G., Chairakaki K., Melas I. Substrate rigidity alters the secreted protein profile and chemotaxis response of fibrosarcoma cells. 444th Wilhelm and Else Heraeus Seminar on Cell Mechanics and 3rd European Meeting on Cell Mechanics Proceedings, Bad Honnef, GERMANY, October Dailiana, ZH, E. Mylona, X. Trepat, M.G. Lagoudakis, and M. Ioannou. Primary human osteosarcoma cell responses to alterations in substrate rigidity. Proceedings of the 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, NV, USA, Mylona E. Cancer cell migration: substrate mechanics and intracellular mechanisms. Proceedings of the American Society of Cell Biology Annual Meeting, p.81, Mylona E. Cancer cell mechanotaxis and intracellular mechanisms. Proceedings of the American Society for Matrix Biology Biennial Meeting, Mylona E., D. Manoussaki, and F. Jetzek. Substrate Rigidity Regulates Multiple Responses of Tumor Cells. Proceedings of the American Society for Cell Biology & European Cytoskeleton Forum Proceedings p. 23, Mylona E., T.S. Mills and G.K. Pavlath. CD44 regulates multiple steps in myogenesis. Proceedings of the American Society for Cell Biology Conference, Mol. Biol. Cell 15 (Suppl. S): 2217, Mylona, E., S.K. Tsivitse, J.M. Peterson, and F.X. Pizza. Myotube-derived neutrophil chemoattractants: Effects of mechanical strain and injury. FASEB J, Mylona, E., T.J. McLoughlin, T. Hornberger, K.A. Esser. Muscle inflammatory cell concentrations following electrically stimulated contraction. FASEB J 16(5): A1138, Page 5 9. Peterson, J.M., T.A. Trappe, E. Mylona, F. White, C.P. Lambert, W.J. Evans, and F.X. Pizza. Ibuprofen and acetaminophen: Effect on muscle inflammation following eccentric contractions. FASEB J 16(5): A1139, Anning, J.H., A.L. Morgan, P.G. Brolinson, P.M. Arnos, E. Mylona, and C. Wilkins. Influence of running and cycling on hydrostatic and oncotic forces associated with plasma volume shifts. Med. Sci. Sports & Exer. 34(5): S113, Tsivitse, S.K., S.J. McGregor, J.M. Peterson, T.J. McLoughlin, E. Mylona, and F.X. Pizza. Muscle inflammatory cell concentrations following down-hill running. Med Sci Sports Exer. 33(5): S120, Kolasinski, S., T.J. McLoughlin, E. Mylona, S.K. Tsivitse, and F.X. Pizza. Effect of ischemic preconditioning on contraction-induced skeletal muscle injury. Med. Sci. Sports Exer. 33(5): S122, Mylona, E., E P.M. Arnos, J.H. Anning, S.A. Albers, A.L. Morgan, and F.F. Andres. Hemodynamic responses to moderate swimming and running during exercise and recovery. Med. Sci. Sports Exer. 31(5): S57, Fahlman, M., E. Mylona, A.L. Morgan, D.J. Boardley, and S.K. Tsivitse. S-IgA following a single bout of exercise: Effects of fitness level and ambient temperature. Med. Sci. Sports Exer. 31(5): S61, Braun, W.A., M.G. Flynn, D. Jacks, T. McLoughlin, J. Sowash, C. Lambert, E. Mylona, and M. Hansen. Effect of indomethacin on NK activity of PBMC. Med. Sci. Sports Exer. 31(5): S61, Anning, J.H., C. Armstong, E. Mylona, S. Norkus, R. Sterner, and F. Andres. Physiological responses during cardiovascular kickboxing: a pilot study. Med. Sci. Sports Exer. 31(5): S110, Arnos, P.M., E. Mylona, J.H. Anning, C. Armstrong, and F.F. Andres. Muscle activation patterns during total-body steady-state exercise. Med. Sci. Sports Exer. 31(5): S193, Lambert, C.P., M.G. Flynn, W.A. Braun, and E. Mylona. The influence of acute submaximal exercise on suppressor cell function in healthy men. Med. Sci. Sports Exer. 30(5): S19, Flynn, M.G., W.A. Braun, J. Armstrong, C.P. Lambert, D. Jacks, J. Yates, and E. Mylona. Effect of oral chondroitin sulfate supplements on muscle soreness, creatine kinase, and markers of inflammation. Med. Sci. Sports Exer. 30(5): S103, Braun, W.A., M.G. Flynn, D. Jacks, T. McLoughlin, J. Sowash, C.P. Lambert, E. Mylona, and M. Hansen. Mechanisms of post-exercise NK suppression. Med. Sci. Sports Exer. 30(5): S175, E. Mylona, D. Grekinis, P. Antoniou, N. Sofiadis, and S. Tokmakidis. Effects of heredity on hemodynamics of pre-adolescent swimmers during maximal exercise and recovery. Exercise and Society, CONFERENCE PRESENTATIONS 1. Mylona E., Alexopoulos L.G., Chairakaki K., Melas I. Substrate rigidity alters the secreted protein profile and chemotaxis response of fibrosarcoma cells. 444th Wilhelm and Else Heraeus Seminar on Cell Mechanics and 3rd European Meeting on Cell Mechanics, Physikzentrum, Bad Honnef, GERMANY, October, Mylona E. Cancer cell mechanotaxis and mechanisms, Cell Biomechanics Wrokshop, Heraklion, HELLAS, Dailiana, ZH; E. Mylona, X. Trepat, M.G. Lagoudakis, and M. Ioannou. Primary human osteosarcoma cell responses to alterations in substrate rigidity. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, NV, USA, Page 6 4. Mylona E. Cancer cell migration: substrate mechanics and intracellular mechanisms. 48 th American Society of Cell Biology Annual Meeting, San Francisco, CA, USA, Mylona E. Cancer cell mechanotaxis and intracellular mechanisms. American Society for Matrix Biology Biennial Meeting, San Diego, CA, USA, Mylona E., E Z.H. Dailiana, X. Trepat, and M.G. Lagoudakis. Substrate Rigidity Dictates Phenotype, Survival, and Mechanics of Primary Human Osteosarcoma Cells, 6 th European Symposium on Biomedical Engineering Symposium, Chania, Crete, HELLAS, Jetzek F., E. Mylona, and D. Manoussaki. Prediction of potential locations of focal adhesions on the contour of adherent cells, Fifth IEEE International Symposium on Biomedical Imaging, Paris, FRANCE, Jetzek F., E. Mylona, and D. Manoussaki. Does Nucleus Morphology in Adherent Cells Correlate with the Spatial Distribution of Focal Adhesions? MCRTN Summer Meeting 2007, Dundee, UK, Mylona E., D. Manoussaki, and F. Jetzek. Substrate Rigidity Regulates Multiple Responses of Tumor Cells. American Society for Cell Biology & European Cytoskeleton Forum Summer Meeting, Dijon, FRANCE, Mylona, E., T.S. Mills, and G.K. Pavlath. CD44 controls multiple steps in myogenesis. Annual Conference of the American Society for Cell Biology, Washington D.C., USA, Mylona, E., S.K. Tsivitse, J.M. Peterson, and F.X. Pizza. Myotube-derived neutrophil chemoattractants: Effects of mechanical strain and injury. Experimental Biology - Annual Meeting of the American Physiological Society, San Diego, USA, Mylona, E., T.J. McLoughlin, T. Hornberger, K. Esser, and F.X. Pizza. Muscle inflammatory cell concentrations following electrically stimulated contractions. Experimental Biology - Annual Meeting of the American Physiological Society, New Orleans, USA, Peterson, J.M, T.A. Trappe, E. Mylona, F.White, C.P. Lambert, W.J. Evans, and F.X. Pizza. Ibuprofen and acetaminophen: Effect on muscle inflammation following eccentric contractions. Experimental Biology - Annual Meeting of the American Physiological Society, New Orleans, USA, Anning, J.H., A.L. Morgan, P.G. Brolinson, P.M. Arnos, E. Mylona, and C. Wilkins. Influence of running and cycling on hydrostatic and oncotic forces associated with plasma volume shifts. Annual Meeting of the American College of Sports Medicine, Saint Louis, USA, Mylona, E., T.J. McLoughlin, T. Hornberger, K. Esser, and F.X. Pizza. Contraction-specific changes in inflammatory cells of rodent skeletal muscle. Regional Meeting of the American College of Sports Medicine, Columbus, OH, USA, Tsivitse, S.K., S.J. McGregor, J.M. Peterson, T.J. McLoughlin, E. Mylona, and F.X. Pizza. Muscle inflammation following down-hill running, Annual Meeting of the American College of Sports Medicine, Baltimore, MD, USA, Kolasinski, S., T.J. McLoughlin, E. Mylona, S.K. Tsivitse, and F.X. Pizza. Effect of ischemic preconditioning on contraction-induced skeletal muscle injury. Annual Meeting of the American College of Sports Medicine, Baltimore, MD, USA, Mylona, E., P.M. Arnos, J.H. Anning, S.A. Albers, A.L. Morgan, and F.F. Andres. Hemodynamic responses to moderate swimming and running during exercise and recovery. Annual Meeting of the American College of Sports Medicine, Seattle, WA, USA, Anning, J.H., C. Armstong, E. Mylona, S. Norkus, R. Sterner, and F. Andres. Physiological responses during cardiovascular kickboxing: a pilot study. Annual Meeting of the American College of Sports Medicine, Seattle, WA, USA Page 7 20. Arnos, P.M., E. Mylona, J.H. Anning, C. Armstrong, and F.F. Andres. Muscle activation patterns during total-body steady-state exercise. Annual Meeting of the American College of Sports Medicine, Seattle, WA, USA Braun, W.A., M.G. Flynn, D. Jacks, T. McLoughlin, J. Sowash, C. Lambert, E. Mylona, and M. Hansen. Effect of Indomethacin on NK activity of PBMC. Annual Meeting of the American College of Sports Medicine, Seattle, WA, USA Lambert, C.P., M.G. Flynn, W.A. Braun, and E. Mylona. The influence of acute submaximal exercise on suppressor cell function in healthy men. Annual Meeting of the American College of Sports Medicine, Orlando, FL, USA Flynn, M.G., W.A. Braun, J. Armstrong, C.P. Lambert, D. Jacks, J. Yates, and E. Mylona. Effect of oral chondroitin sulfate supplements on muscle soreness, creatine kinase, and markers of inflammation. Annual Meeting of the American College of Sports Medicine, Orlando, FL, USA, Braun, W.A., M.G. Flynn, D. Jacks, T. McLoughlin, J. Sowash, C.P. Lambert, E. Mylona, and M. Hansen. Mechanisms of post-exercise NK suppression. Annual Meeting of the American College of Sports Medicine, Orlando, FL, USA, E. Mylona, D. Grekinis, P. Antoniou, N. Sofiadis, and S. Tokmakidis. Effects of heredity on hemodynamics of pre-adolescent swimmers during maximal exercise and recovery. International Congress on Physical Education and Exercise Science, Komotini, HELLAS, CONFERENCE PARTICIPATION WITHOUT PRESENTATION 1. Conference on Invasion and Metastasis, Max Delbrück Centrum für Molekulare Medizin, Berlin- Buch, GERMANY, Workshop on Cancer Modelling and Therapeutic Innovation: From Theory to Clinic, Ecole Normale Supérieure de Lyon, Lyon, FRANCE, Second Summer School, Marie Curie Research Training Network: Modelling Mathematical Methods and Computer Simulation of Tumour Growth and Therapy, Kolymbari, Crete, HELLAS, Euroconference in Biomathematics, Which Mathematics for Biology?, Anogia, Crete, HELLAS, Annual Meeting of the American College of Sports Medicine, Indianapolis, IN, USA, Annual Meeting of the American College of Sports Medicine, Denver, CO, USA, INVITED TAL ALKS 19/5/2008 Characterizing cancer cell response (with D. Manoussaki) CancerSim2008, Euroconference on Modeling and
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